Instructor: Ulrich G. Mueller (umueller@mail.utexas.edu)
Teaching Assistant: Rachelle Adams (rmmadams@mail.utexas.edu)
DATE TOPIC
Sept 3 Overview and lab organization
Sept 9 CTAB DNA extraction, Chelex extraction, column extraction
Sept 10 CTAB DNA extraction (continued)
Sept 16 Agarose gel electrophoresis of genomic DNA; DNA quantification;
Sept 17 Polymerase Chain Reaction (PCR)
Sept 23 PCR product electrophoresis
Sept 24 RFLP 1: Restriction enzyme digest
Sept 30 RFLP 2: RFLP analyses
Oct 1 RAPD-PCR (Rapid Amplified Polymorphic DNA PCR)
Oct 7 ISSR-PCR (Inter Simple Sequence Repeat PCR)
Oct 8 RAPD and ISSR analyses
Oct 14 Microsatellite development 1: Cloning and gridding
Oct 15 Microsatellite development 2: PCR of clones
Oct 21 Microsatellite development 3 / Sequencing 1: Selecting template
Oct 22 Microsatellite development 4 / Sequencing 2: Cleaning template (PEGing)
Oct 28 Microsatellite development 5 / Sequencing 3: Cycle sequencing reaction
Oct 29 Microsatellite development 6 / Sequencing 4: Sephadex cleaning of reactions
Nov 4 Microsatellite development 7 / Sequencing 5: Automated sequencing on
ABI 3100 automated sequencer (Mueller Lab, Patterson 634)
Nov 5 Microsatellite development 8: Primer design
Nov 11 Sequencing 6: Sequence editing and alignment; Genbank accessions
Nov 12 AFLP: Restriction-ligation of genomic DNA
Nov 18 AFLP: Pre-selective amplification
Nov 19 AFLP: Selective amplification
Nov 25 AFLP: Analysis on ABI 3100 automated genotyper (Mueller Lab, Patterson 634)
Nov 26 AFLP: Analysis using Genotyper sofware
Dec 2 Student presentations
Dec 3 Student presentations (continued)