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John Sisson
John Sisson

Assistant Professor

Ph.D. Stanford University School of Medicine

Sisson@icmb.utexas.edu

Office 219 Patterson Labs
(512) 475-7561

Sisson lab home page

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The Sisson lab is interested in the molecular mechanisms that control how cells form and differentiate in early animal embryos. Our research is currently focused on elucidating the molecular mechanisms of two processes that control the formation of the first polarized epithelial cell layer in early Drosophila embryos: Golgi-dependent membrane secretion and Fragile X mental retardation protein-dependent translational regulation. We study these processes using an integrated experimental approach that combines the advantages of biochemistry, genetics, molecular biology and live embryo imaging.


Selected Publications


Dagani, G. T., Monzo, K., Fakhoury, J. R., Chen, C. C., Sisson, J. C., and X. Zhang (2007). Microfluidic self-assembly of live Drosophila embryos for versatile high-throughput analysis of embryonic morphogenesis. Biomedical Microdevices (in press).

Monzo, K., Papoulas, O., Cantin, G. T., Wang, Y., Yates, J. R., and J. C. Sisson (2006). Fragile X mental retardation protein controls trailer hitch expression and cleavage furrow formation in Drosophila embryos. Proc. Natl. Acad. Sci. 103(48): 18160-18165.

Papoulas, O., Hays, T. S., and J. C. Sisson (2005). The golgin Lava Lamp mediates dynein-based Golgi movements during Drosophila cellularization. Nature Cell Biology 7(6): 612-618.

Beronja, S., Laprise, P., Papoulas, O., Pellikka, M., Sisson, J. C., and U. Tepass. (2005). Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells. Journal of Cell Biology 169(4): 635-646.

Kidd, T., Abu-Shumays, R., Katzen, A., Sisson, J. C., Jimenez, G., Pinchin, S., Sullivan, W., and D. Ish-Horowicz (2005). Maternal and zygotic requirements for the e subunit of mitochondrial ATP synthase in the mitotic cycles of the Drosophila syncytial blastoderm and larva. Genetics 170: 697-708.

Zarnescu, D., Jin, P., Betschinger, J., Nakamoto, M., Wang, Y., Dockendorff, T., Feng, Y., Jongens, T., Sisson, J. C., Knoblich, J., Warren, S., and K. Moses (2005). Fragile X protein functions with Lgl and the PAR complex in flies and mice. Developmental Cell 8: 43-52.

Royou, A., Field, C., Sisson, J. C., Sullivan, W., and R. Karess (2004). Reassessing the role and dynamics of nonmuscle myosin II during furrow formation and cellularization in the early Drosophila embryo. Molecular Biology of the Cell 15: 838-850.

Sisson, J. C., Field, C., Ventura, R., Royou A., and W. Sullivan (2000).  Lava Lamp, a Novel Peripheral Golgi Protein, is Required for Drosophila Cellularization.  Journal of Cell Biology 151: 905-917.

Sisson, J. C. (2000).  Culturing Large Populations of Drosophila for Protein Biochemistry.  Drosophila Protocols: A Laboratory Manual, eds. W. Sullivan, M. Ashburner, and S. Hawley (Cold Spring Harbor Laboratory Press).

Sharp, D. J., Yu, K. R., Sisson, J. C., Sullivan, W., and Scholey, J. M. (1999). Antagonistic Microtubule-Sliding Motors Position Mitotic Centrosomes in Drosophila Early Embryos.  Nature Cell Biology 1: 51-54.

Sisson, J. C., Rothwell, W., and Sullivan, W. (1999).  Cytokinesis: Lessons from Rappaport and the Drosophila Blastoderm Embryo.  Cell Biology International 23(12):871-876.

Sisson, J. C., Ho, K. S., Suyama, K., and Scott, M. P. (1997) Costal2, a Novel Kinesin-Related Protein in the Hedgehog Signaling Pathway.  Cell 90: 235-245.

Robbins, D. J., Nybbaken, K. E., Kobayashi, R., Sisson, J. C., Bishop, J. M., and Thérond, P. P. (1997).  Hedgehog Elicits Signal Transduction by Means of a Large Complex Containing the Kinesin-Related Protein Costal2.  Cell 90: 225-234.

 
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