Hawkes Lab Protocols

Molecular microbial ecology

• Nucleic acid extraction - single samples

• Nucleic acid extraction - 96 well plates

• microplate template1

• microplate template2

• topoTA cloning

• common PCR primers

• PCR worksheet 96 well plate

• PCR worksheet single samples

Soil microbial abundance and activity

• Glomalin extraction

• Ergosterol extraction

• Soil chitinase assay

• Licor modification for small volume CO2 measurement

• CLPP

Soil biogeochemistry

• KCl extraction for inorganic N

• Persulfate digestion for total N

• CFDE for microbial biomass C & N

• N2 fixation by acetylene reduction

• N2 fixation using 15N2 tracers

• 15N pool dilution

• 15N pool dilution worksheet