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| Jerry
Brand |
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Professor
Ph.D. Purdue
University
jbrand@mail.utexas.edu
Office Bio 321
(512) 471-1589
Home
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| Jerry Brand
is Director of the Culture Collection of Algae at the University
of Texas at Austin (UTEX): web site www.utex.org.
Much of his research involves the development of methods that optimize
the efficiency of culturing living algae under laboratory conditions
while stabilizing their genetic composition. Cryopreservation,
the indefinite storage of living cells or whole organisms at ultra-cold
temperatures, has been studied extensively by Dr. Brand and his associates,
utilizing algal cultures in the UTEX Collection. This work has led
to the development of protocols that allow over 1,300 different kinds
of algae, approximately 2/3 of the strains in the UTEX Collection,
to be cryopreserved. Current work is directed toward the investigation
of reasons that some algal strains are recalcitrant to cryopreservation,
in order to develop new protocols that expand the range of organisms
that can be successfully stored under liquid nitrogen. In particular,
causes of high mortality when algal strains are frozen at high culture
density are being investigated. Collaborative work with Dr. Kenneth
Diller and his associates in the Department of Biomedical Engineering
at UT-Austin involves an investigation of the relative effectiveness
of dimethyl sulfoxide and methanol as cryoprotective agents. The long-term
goal of Jerry Brand's research is to be able to stabilize the genetic
integrity of virtually all strains of algae by storing living cultures
at ultra-low temperatures, and to be able to apply new methods toward
the successful cryopreservation of other organisms such as endangered
plant species. |
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Selected Publications
CRYOPRESERVATION OF CHLAMYDOMONAS REINHARDTII (Chlorophyta) by Alexandra
L. M. Crutchfield, Kenneth R. Diller and Jerry J. Brand. Eur. J. Phycol.
34: 43-52 (1999).
ALGAE PERMEABILITY TO ME2SO FROM -3°C TO +23°C. Jocelyn Y. Tanaka,
John R. Walsh, Kenneth R. Diller, Jerry J. Brand and Shanti J. Aggarwal.
Cryobiology 42: 286-300 (2001)
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