| Research focuses on three main areas: (i) development,
and evolutionary changes in development, using pigment patterns as
a model system; (ii) the genetic bases of metamorphosis; and (iii)
comparative genomics. Brief descriptions of these research interests,
and example photographs, are provided below. For all of these areas
we use two main groups of organisms: fishes (including the zebrafish
Danio rerio) and salamanders, each of which undergo a metamorphosis
sometime during their development, thereby transforming an embryonic
or larval phenotype into an adult phenotype. We also use a variety
of methods, including: molecular and classical genetics; mutant screens;
DNA sequencing; in situ hybridization analysis of gene expression;
light and electron microscopy; cell lineage tracing; microsurgical
manipulations; and phylogenetic comparative methods. Students joining
the lab are encouraged to work on developmental, genetic, evolutionary,
or behavioral aspects of pigment patterns or other morphological
characters, and ideally to integrate multiple approaches into their
research.
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Selected Publications
Parichy, D.M. and Turner, J.M. 2003. Temporal and cellular
requirements for fms signaling during zebrafish adult pigment pattern
development. Development 130:817-833.
Parichy, D.M. 2001. Homology and evolutionary novelty in the deployment
of extracellular matrix molecules during pigment pattern formation in salamanders
(Taricha, Ambystoma). Molecular and Developmental Evolution (Journal of Experimental
Zoology) 291:13-24.
Parichy, D.M., and Johnson, S.L. 2001. Zebrafish hybrids suggest genetic
mechanisms of pigment pattern diversification in Danio. Development, Genes and
Evolution 211:319-328.
Voss, S.R., Smith, J.J., Gardiner, D., and Parichy, D.M. 2001. Conserved
vertebrate chromosome segments in the large salamander genome. Genetics 158:735-746.
Parichy, D. M., Mellgren, E. M., Rawls, J. F., Lopes, S.S., Kelsh, R.N.,
and Johnson, S. L. 2000. Mutational analysis of endothelin receptor b1 (rose)
during neural crest and pigment pattern development in the zebrafish, Danio rerio.
Developmental Biology, 227:294–306.
Parichy, D. M. 2000. Homology and evolutionary novelty in the deployment
of extracellular matrix molecules dur-ing pigment pattern formation in salamanders
(Taricha, Ambystoma). Molecular and Developmental Evolution (Journal of Experimental
Zoology), in press.
Parichy, D. M., Ransom, D. G., Paw, B., Zon, L. and Johnson, S. L. 2000.
An orthologue of the kit-related gene fms is required for development of neural
crest–derived xanthophores and a subpopulation of adult melanocytes in
the zebrafish, Danio rerio. Development, 127:3031–3044.
Parichy, D. M., Rawls, J. F., Whitfield, T., Pratt, S. J. and Johnson,
S. L. 1999. Zebrafish sparse corresponds to an orthologue of c-kit and is required
for the morphogenesis of a subpopulation of melanocytes, but is not essential
for hematopoiesis or primordial germ cell development. Development 126:3425–3436.
Parichy, D. M. 1998. Experimental analysis of character coupling across
a complex life cycle: pigment pattern metamorphosis in the tiger salamander,
Ambystoma tigrinum tigrinum. Journal of Morphology 237:53–67.
Parichy, D. M. 1996. Pigment patterns of larval salamanders (Ambystomatidae,
Salamandridae): the role of the lateral line sensory system and the evolution
of pattern-forming mechanisms. Developmental Biology 175:265–282
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